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human serum, pregnant, 1st trimester  (Golden West Biologicals)
99
Golden West Biologicals human serum, pregnant, 1st trimester
Human Serum, Pregnant, 1st Trimester, supplied by Golden West Biologicals, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human serum, pregnant, 1st trimester/product/Golden West Biologicals
Average 99 stars, based on 1 article reviews
human serum, pregnant, 1st trimester - by Bioz Stars, 2026-04
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primescript ii 1st strand cdna synthesis kit  (TaKaRa)
97
TaKaRa primescript ii 1st strand cdna synthesis kit
Primescript Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primescript ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-04
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strand cdna synthesis kit  (TaKaRa)
99
TaKaRa strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/strand cdna synthesis kit/product/TaKaRa
Average 99 stars, based on 1 article reviews
strand cdna synthesis kit - by Bioz Stars, 2026-04
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human serum, pregnant, 1st trimester, pooled  (Golden West Biologicals)
99
Golden West Biologicals human serum, pregnant, 1st trimester, pooled
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Human Serum, Pregnant, 1st Trimester, Pooled, supplied by Golden West Biologicals, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human serum, pregnant, 1st trimester, pooled/product/Golden West Biologicals
Average 99 stars, based on 1 article reviews
human serum, pregnant, 1st trimester, pooled - by Bioz Stars, 2026-04
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hiscript iii 1st strand cdna synthesis kit +gdna wiper  (Vazyme Biotech Co)
99
Vazyme Biotech Co hiscript iii 1st strand cdna synthesis kit +gdna wiper
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Hiscript Iii 1st Strand Cdna Synthesis Kit +Gdna Wiper, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiscript iii 1st strand cdna synthesis kit +gdna wiper/product/Vazyme Biotech Co
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hiscript iii 1st strand cdna synthesis kit +gdna wiper - by Bioz Stars, 2026-04
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1st strand cdna synthesis kit  (Boehringer Mannheim)
90
Boehringer Mannheim 1st strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
1st Strand Cdna Synthesis Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
1st strand cdna synthesis kit - by Bioz Stars, 2026-04
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hiscript ii 1st strand cdna synthesis kit  (Vazyme Biotech Co)
96
Vazyme Biotech Co hiscript ii 1st strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Hiscript Ii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiscript ii 1st strand cdna synthesis kit/product/Vazyme Biotech Co
Average 96 stars, based on 1 article reviews
hiscript ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-04
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mirna 1st strand cdna synthesis kit  (Vazyme Biotech Co)
98
Vazyme Biotech Co mirna 1st strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Mirna 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirna 1st strand cdna synthesis kit/product/Vazyme Biotech Co
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mirna 1st strand cdna synthesis kit - by Bioz Stars, 2026-04
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mmlv reverse transcriptase 1st strand cdna synthesis kit  (Lucigen Corp)
90
Lucigen Corp mmlv reverse transcriptase 1st strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Mmlv Reverse Transcriptase 1st Strand Cdna Synthesis Kit, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmlv reverse transcriptase 1st strand cdna synthesis kit - by Bioz Stars, 2026-04
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cdna synthesis kit  (TaKaRa)
99
TaKaRa cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hifair iii 1st strand cdna synthesis supermix for qpcr (gdna digester plus)  (Yeasen Biotechnology)
90
Yeasen Biotechnology hifair iii 1st strand cdna synthesis supermix for qpcr (gdna digester plus)
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Hifair Iii 1st Strand Cdna Synthesis Supermix For Qpcr (Gdna Digester Plus), supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hifair iii 1st strand cdna synthesis supermix for qpcr (gdna digester plus)/product/Yeasen Biotechnology
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hiscript 1st strand cdna synthesis kit  (Vazyme Biotech Co)
96
Vazyme Biotech Co hiscript 1st strand cdna synthesis kit
Figure 6. Southern blot analysis of PCR products obtained from <t>total</t> <t>RNA</t> isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml <t>cDNA</t> mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.
Hiscript 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 6. Southern blot analysis of PCR products obtained from total RNA isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Journal: Stem cells (Dayton, Ohio)

Article Title: Adenovirus mediated alpha interferon (IFN-alpha) gene transfer into CD34+ cells and CML mononuclear cells.

doi: 10.1002/stem.150386

Figure Lengend Snippet: Figure 6. Southern blot analysis of PCR products obtained from total RNA isolated from CD34+ cells transfected with AdCMV-IFN- α gene. Lane 1, PCR products amplified from nontransfected (control) CD34+ cell RNA; lane 2, RNA obtained from CD34+ cells transfected with AdCMV-IFN-α after 24 h; lanes 3 and 4, RNA from CFU-GM clones after 12 and 14 days of culture, respectively; lanes 5 and 6, RNA from BFU-E clones after 12 and 14 days of culture. Total RNA was extracted from CD34+ cells, BFU-E or CFU-GM clones. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction volume of 25 ml. Aliquots of the reaction mixture (10 ml) were subjected to electrophoresis in a 0.8% agarose (Sigma; St. Louis, MO) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, (gift of Dr. Asano) using α-32d-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Article Snippet: RNA is reverse-transcribed using the first strand cDNA synthesis kit from Clontech, Inc. (Palo Alto, CA).

Techniques: Southern Blot, Isolation, Transfection, Amplification, Control, Clone Assay, Electrophoresis

Figure 7. Southern blot analysis of PCR products obtained from total RNA isolated from CML hematopoietic stem cells (BMMNC) transfected with AdCMV-IFN-α gene. Lane 1, PCR products amplified from nontransfected (control) cell RNA; lane 2, RNA obtained from cells transfected with AdCMV-IFN-α after 24 h; lane 3, RNA from CFU-GM clones after 12 days; lane 4, CFU-GM clones after 14 days of culture; lane 5, no RNA was added; and lane 6, positive control. Total RNA was extracted from stem cells or CFU-GM clones by one single extraction. cDNA was synthesized from total RNA. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction vol- ume of 25 ml. Aliquots of the reaction mixture (10 ml) were sub- jected to electrophoresis in a 0.8% agarose (Sigma) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, using α-32P-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Journal: Stem cells (Dayton, Ohio)

Article Title: Adenovirus mediated alpha interferon (IFN-alpha) gene transfer into CD34+ cells and CML mononuclear cells.

doi: 10.1002/stem.150386

Figure Lengend Snippet: Figure 7. Southern blot analysis of PCR products obtained from total RNA isolated from CML hematopoietic stem cells (BMMNC) transfected with AdCMV-IFN-α gene. Lane 1, PCR products amplified from nontransfected (control) cell RNA; lane 2, RNA obtained from cells transfected with AdCMV-IFN-α after 24 h; lane 3, RNA from CFU-GM clones after 12 days; lane 4, CFU-GM clones after 14 days of culture; lane 5, no RNA was added; and lane 6, positive control. Total RNA was extracted from stem cells or CFU-GM clones by one single extraction. cDNA was synthesized from total RNA. IFN-α transcripts were amplified by PCR using 2 ml of 100 ml cDNA mixture of a total reaction vol- ume of 25 ml. Aliquots of the reaction mixture (10 ml) were sub- jected to electrophoresis in a 0.8% agarose (Sigma) gel. Nucleic acids were transferred to nitrocellulose by blotting and probed with IFN-α cDNA probes, using α-32P-CTP. Filters were exposed to x-ray for 48 h at –80°C.

Article Snippet: RNA is reverse-transcribed using the first strand cDNA synthesis kit from Clontech, Inc. (Palo Alto, CA).

Techniques: Southern Blot, Isolation, Transfection, Amplification, Control, Clone Assay, Positive Control, Extraction, Synthesized, Electrophoresis